Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein
Identifieur interne : 003B60 ( Main/Exploration ); précédent : 003B59; suivant : 003B61Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein
Auteurs : Ying-Xim Tan [Singapour] ; Timothy H. P. Tan [Singapour] ; Marvin J.-R. Lee [Singapour] ; Puay-Yoke Tham [Singapour] ; Vithiagaran Gunalan [Singapour] ; Julian Druce [Australie] ; Chris Birch [Australie] ; Mike Catton [Australie] ; NAI YANG FU [Singapour] ; Victor C. Yu [Singapour] ; Yee-Joo Tan [Singapour]Source :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules Vero, Données de séquences moléculaires, Délétion de gène, Humains, Immunoprécipitation, Mitochondries (métabolisme), Mutation, Protéine bcl-X (métabolisme), Protéines de la matrice virale (métabolisme), Protéines de la matrice virale (physiologie), Protéines virales (métabolisme), Protéines virales (physiologie), Similitude de séquences d'acides aminés, Structure tertiaire des protéines, Séquence d'acides aminés.
- MESH :
- métabolisme : Mitochondries, Protéine bcl-X, Protéines de la matrice virale, Protéines virales.
- physiologie : Protéines de la matrice virale, Protéines virales.
- Pascal (Inist)
- Animaux, Apoptose, Cellules Vero, Coronavirus, Apoptose, Données de séquences moléculaires, Délétion de gène, Humains, Immunoprécipitation, Mort cellulaire, Mutation, Protéine, Similitude de séquences d'acides aminés, Structure tertiaire des protéines, Séquence d'acides aminés, Virologie, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Apoptosis, Cell death, Chlorocebus aethiops, Coronavirus, Gene Deletion, Humans, Immunoprecipitation, Mitochondria (metabolism), Molecular Sequence Data, Mutation, Protein, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Severe acute respiratory syndrome, Vero Cells, Viral Matrix Proteins (metabolism), Viral Matrix Proteins (physiology), Viral Proteins (metabolism), Viral Proteins (physiology), Virology, bcl-X Protein (metabolism).
- MESH :
- chemical , metabolism : Viral Matrix Proteins, Viral Proteins, bcl-X Protein.
- metabolism : Mitochondria.
- chemical , physiology : Viral Matrix Proteins, Viral Proteins.
- Amino Acid Sequence, Animals, Apoptosis, Chlorocebus aethiops, Gene Deletion, Humans, Immunoprecipitation, Molecular Sequence Data, Mutation, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Vero Cells.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. In this study, we show that the overexpression of Bcl-XL, a prosurvival member of the Bcl-2 family, blocks 7a-induced apoptosis, suggesting that the mechanism for apoptosis induction by 7a is at the level of or upstream from the Bcl-2 family. Coimmunoprecipitation experiments showed that 7a interacts with Bcl-XL and other prosurvival proteins (Bcl-2, Bcl-w, Mcl-1, and Al) but not with the proapoptotic proteins (Bax, Bak, Bad, and Bid). A good correlation between the abilities of 7a deletion mutants to induce apoptosis and to interact with Bcl-XL was observed, suggesting that 7a triggers apoptosis by interfering directly with the prosurvival function of Bcl-XL. Interestingly, amino acids 224 and 225 within the C-terminal transmembrane domain of Bcl-XL are essential for the interaction with the 7a protein, although the BH3 domain of Bcl-XL also contributes to this interaction. In addition, fractionation experiments showed that 7a colocalized with Bcl-XL at the endoplasmic reticulum as well as the mitochondria, suggesting that they may form complexes in different membranous compartments.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000363
- to stream PascalFrancis, to step Curation: 000626
- to stream PascalFrancis, to step Checkpoint: 000371
- to stream Main, to step Merge: 003D18
- to stream PubMed, to step Corpus: 001E46
- to stream PubMed, to step Curation: 001E46
- to stream PubMed, to step Checkpoint: 001D81
- to stream Ncbi, to step Merge: 001929
- to stream Ncbi, to step Curation: 001929
- to stream Ncbi, to step Checkpoint: 001929
- to stream Main, to step Merge: 003815
- to stream Main, to step Curation: 003B60
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-X<sub>L</sub> protein</title><author><name sortKey="Tan, Ying Xim" sort="Tan, Ying Xim" uniqKey="Tan Y" first="Ying-Xim" last="Tan">Ying-Xim Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0315403</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0315403 INIST</idno><idno type="RBID">Pascal:07-0315403</idno><idno type="wicri:Area/PascalFrancis/Corpus">000363</idno><idno type="wicri:Area/PascalFrancis/Curation">000626</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000371</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000371</idno><idno type="wicri:doubleKey">0022-538X:2007:Tan Y:induction:of:apoptosis</idno><idno type="wicri:Area/Main/Merge">003D18</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:17428862</idno><idno type="wicri:Area/PubMed/Corpus">001E46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E46</idno><idno type="wicri:Area/PubMed/Curation">001E46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E46</idno><idno type="wicri:Area/PubMed/Checkpoint">001D81</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001D81</idno><idno type="wicri:Area/Ncbi/Merge">001929</idno><idno type="wicri:Area/Ncbi/Curation">001929</idno><idno type="wicri:Area/Ncbi/Checkpoint">001929</idno><idno type="wicri:doubleKey">0022-538X:2007:Tan Y:induction:of:apoptosis</idno><idno type="wicri:Area/Main/Merge">003815</idno><idno type="wicri:Area/Main/Curation">003B60</idno><idno type="wicri:Area/Main/Exploration">003B60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-X<sub>L</sub> protein</title><author><name sortKey="Tan, Ying Xim" sort="Tan, Ying Xim" uniqKey="Tan Y" first="Ying-Xim" last="Tan">Ying-Xim Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Apoptosis</term><term>Cell death</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Gene Deletion</term><term>Humans</term><term>Immunoprecipitation</term><term>Mitochondria (metabolism)</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Protein</term><term>Protein Structure, Tertiary</term><term>Sequence Homology, Amino Acid</term><term>Severe acute respiratory syndrome</term><term>Vero Cells</term><term>Viral Matrix Proteins (metabolism)</term><term>Viral Matrix Proteins (physiology)</term><term>Viral Proteins (metabolism)</term><term>Viral Proteins (physiology)</term><term>Virology</term><term>bcl-X Protein (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Délétion de gène</term><term>Humains</term><term>Immunoprécipitation</term><term>Mitochondries (métabolisme)</term><term>Mutation</term><term>Protéine bcl-X (métabolisme)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines de la matrice virale (physiologie)</term><term>Protéines virales (métabolisme)</term><term>Protéines virales (physiologie)</term><term>Similitude de séquences d'acides aminés</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Matrix Proteins</term><term>Viral Proteins</term><term>bcl-X Protein</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Mitochondries</term><term>Protéine bcl-X</term><term>Protéines de la matrice virale</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines de la matrice virale</term><term>Protéines virales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Viral Matrix Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Apoptosis</term><term>Chlorocebus aethiops</term><term>Gene Deletion</term><term>Humans</term><term>Immunoprecipitation</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Protein Structure, Tertiary</term><term>Sequence Homology, Amino Acid</term><term>Vero Cells</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Coronavirus</term><term>Apoptose</term><term>Données de séquences moléculaires</term><term>Délétion de gène</term><term>Humains</term><term>Immunoprécipitation</term><term>Mort cellulaire</term><term>Mutation</term><term>Protéine</term><term>Similitude de séquences d'acides aminés</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. In this study, we show that the overexpression of Bcl-X<sub>L</sub>, a prosurvival member of the Bcl-2 family, blocks 7a-induced apoptosis, suggesting that the mechanism for apoptosis induction by 7a is at the level of or upstream from the Bcl-2 family. Coimmunoprecipitation experiments showed that 7a interacts with Bcl-X<sub>L</sub> and other prosurvival proteins (Bcl-2, Bcl-w, Mcl-1, and Al) but not with the proapoptotic proteins (Bax, Bak, Bad, and Bid). A good correlation between the abilities of 7a deletion mutants to induce apoptosis and to interact with Bcl-X<sub>L</sub> was observed, suggesting that 7a triggers apoptosis by interfering directly with the prosurvival function of Bcl-X<sub>L</sub>. Interestingly, amino acids 224 and 225 within the C-terminal transmembrane domain of Bcl-X<sub>L</sub> are essential for the interaction with the 7a protein, although the BH3 domain of Bcl-X<sub>L</sub> also contributes to this interaction. In addition, fractionation experiments showed that 7a colocalized with Bcl-X<sub>L</sub> at the endoplasmic reticulum as well as the mitochondria, suggesting that they may form complexes in different membranous compartments.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Singapour</li></country></list><tree><country name="Singapour"><noRegion><name sortKey="Tan, Ying Xim" sort="Tan, Ying Xim" uniqKey="Tan Y" first="Ying-Xim" last="Tan">Ying-Xim Tan</name></noRegion><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name></country><country name="Australie"><noRegion><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name></noRegion><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003B60 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003B60 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:07-0315403
|texte= Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein
}}
| This area was generated with Dilib version V0.6.33. |  |